What Is Cryogenic Gas?

A Cryogenic liquid is liquefied gases that is maintained in the liquid state at low temperatures. Different cryogenic gases become liquids at different of temperatures and pressures The two common properties of the cryogens are that in the liquid state, they are extremely cold, and very small volumes of the liquid cryogen can expand into very large volumes of cryogenic gas.

A Supplier of Cryogenic Gases

CryoService supply liquid gases to a wide range of cryogenic applications in the Science, Industry and Leisure sectors.

With an extensive network of Cryogenic gas delivery depots across the UK and a fleet optimised for city centre and small bulk liquid gas activities, CryoService have the breadth of cryogenic and liquid gas  product range and local support to support the most exacting cryopreservation or liquid nitrogen needs.

Liquid Nitrogen

• CryoService are one the leading liquid nitrogen suppliers in the UK. Liquid nitrogen supply is made in quantities from desk flasks and non-pressurised dewars up to small bulk vessels of 6000 – 10,000 litres.

Liquid Argon & Liquid Oxygen

• CryoService are a specialist liquid argon and oxygen supplier for industrial and scientific applications and these products are available in pressurised vessels from 180 – 10,000 litre capacities.

Liquid Carbon Dioxide

• CryoService supply liquid carbon dioxide in a range of packages, from cylinders (including gas offtake and dip tube configurations) through to bulk supply of up to 10 tonne capacity.

Our liquid gas supply is supported by our extensive Engineering Services offerings, including design, installation, maintenance and training.

To find out more contact one of our experienced Sales Team at CryoService Ltd:

General and Sales Telephone Enquiries:

01905 758200

Fax:

01905 754060

Email:

info@cryoservice.co.uk

Awareness of Oxygen Depletion in Liquid Nitrogen Applications

LIN filling delivery

All I need is the air that I breathe….

Like most living organisms, humans have a few basic requirements for sustaining life – and sadly beer and curry are actually not on that list! However oxygen is essential for life and our requirements are actually quite specific and relate to the atmosphere that we as a species have developed in. Any depletion of the oxygen level in air must be treated with concern, and as a minimum the concentration in the workplace should be maintained above 19.5%. Crucially, atmospheres containing less than 18% oxygen are potentially dangerous and suitable protection methods should be adopted (e.g. the use of breathing apparatus).

Asphyxiation as a result of oxygen depletion can take place on a gradual or sudden basis, depending upon the extent of the depletion. It is a little known fact that inhalation of a gas containing virtually no oxygen can result in immediate unconsciousness and rapid death. The symptoms associated with oxygen depleted atmospheres are detailed in Table 1.

Table 1 – Asphyxia Symptoms for Low Oxygen Levels

Oxygen Content of Air	Signs and Symptoms of Asphyxia
18% – 19.5%	May affect physical and intellectual performance without person’s knowledge.
15% – 18%	Decreased ability to work strenuously. May impair co-ordination and may induce symptoms in persons with coronary, pulmonary, or circulatory problems.
12% – 15%	Respiration deeper, increased pulse rate, and impaired co-ordination, perception and judgment.
10% – 12%	Further increase in rate and depth of respiration, further increase in pulse rate, performance failure, giddiness, poor judgment, blue lips.
8% – 10%	Mental failure, nausea, vomiting, fainting, ashen face, blue lips.
6% – 8%	Loss of consciousness within a few minutes, resuscitation possible if carried out immediately.
0% – 6%	Loss of consciousness almost immediate, death ensues, brain damage even if rescued.

Liquid nitrogen – the great cryogen

Liquid nitrogen is used in extensively across the World, particularly for its excellent cryogenic properties. Produced from the liquefaction of air, it is colourless, odourless and exists at temperature of -196^oC at atmospheric pressure. Liquid nitrogen is the preferred method of cryogenic storage, for example in the preservation of biological samples, as unlike electrical freezers it relatively cheap to purchase, does not rely on electricity (and is not susceptible to electrical outages) and has low running costs. It is supplied via specialised road tanker into insulated storage vessels, ranging from non-pressurised dewars and desks flasks to pressurised tanks for mobile or static storage.

Liquid nitrogen is also very safe under normal usage and many people associate the extreme cold temperature as the main source of risk. However, when Liquid Nitrogen evaporates (e.g. through spillage) it undergoes a large volume expansion as it returns to the gaseous form – one litre of liquid nitrogen produces approximately 680 litres of nitrogen gas! This expansion ratio will quickly displace the atmosphere within a confined space and can cause oxygen depletion if control measures are not in place.

Insert photo of tank types “Typical small mobile Liquid Nitrogen vessels”

To find out more about Liquid Nitrogen please visit the CryoService Liquid Nitrogen page.

Providing early warning

Whilst fully-integrated “cryorooms” are discussed later, one of the most critical aspects of safety awareness and enhancement in these applications is the correct use of gas detection equipment, in the form of oxygen depletion monitoring.

A typical oxygen depletion monitor consists of an electrochemical sensor which generates a small electrical signal in proportion to the concentration of oxygen present. This sensor signal is then amplified to display the oxygen level on either a portable or fixed-point instrument. Oxygen sensors are typically calibrated in air (having first been zeroed using 100% nitrogen) so that 20.9% volume oxygen is displayed in clean-air environments. Oxygen depletion alarms are usually set at 19% and 17% volume.

Portable instruments are typically worn on breast pockets (i.e. within the vicinity of the breathing zone) to provide protection to personnel. Staff working within an area where an oxygen depletion risk exists are trained to evacuate immediately in the event of their portable monitor producing a low oxygen alarm.

Fixed-point oxygen monitoring systems utilise one or more oxygen sensors installed in the vicinity of potential nitrogen leak sources (ie near vessels, flanges, valves etc), which are connected to a control panel. The sensors permanently monitor the area, and the control panel displays the gas levels and provides alarms in the event of a sensor reporting a reduced concentration of oxygen. Control panels can also be used to take executive actions such as closing solenoid valves to prevent further nitrogen releases. Fixed systems provide the significant advantage of warning of a reduced level of oxygen before personnel enter an area.

Insert picture of Crowcon personal and fixed monitors

To understand more about oxygen depletion system, go to http://www.crowcon.com/

Liquid Nitrogen Case Study – “WORKERS AT ONE OF THE UK’S LARGEST VACUUM TEST CHAMBERS KEPT SAFE WITH CROWCON GAS DETECTORS”

To protect workers from the danger of depleted oxygen levels, the Rutherford Appleton Laboratory has recently installed four Crowcon gas detectors and a Gasmaster control panel. The Gasmaster control panel displays oxygen levels in all the testing cleanrooms simultaneously on a large, LCD display, allowing full system status checks at a glance.

The Crowcon units are installed in the Assembly Integration and Verification (AIV) Facility’s suite of cleanrooms, where spacecraft and satellite components are subjected to extreme temperature and vacuum conditions. The facility has a number of vacuum chambers and other testing laboratories. The largest vacuum chamber – one of the biggest in the UK – is highly versatile and can simulate near-space conditions with temperatures from -196^oC to + 150 ^oC.

The chambers are returned to atmospheric pressure by introducing nitrogen gas. It can take up to four hours to return the largest chamber to atmospheric pressure, and if nitrogen leaked out during this time, it could result in rapid oxygen depletion in the laboratory. The oxygen sensors ensure that should this happen, the Gasmaster control panel will instantly inform controllers which detector has been activated with a visual signal such as “Vacuum chamber 1″, as well as activating audible/visual alarm devices.

“Our air conditioning system changes the air up to six times an hour so if there ever was a nitrogen leak, the aircon would normally take care of it,” commented technician Dave Rippington. “The Crowcon systems are really in place as part of a ‘belt and braces’ approach, ensuring our workers are safe in the highly unlikely event that there was both a nitrogen leak and the air-con system failed.”

Insert picture of RAL.

Best practise Liquid Nitrogen control measures

The main application considered here is biological sample storage within typical research, academic and hospital environments and there are effectively two infrastructures in which this product is used.

The first is the purpose-designed “cryoroom”, a dedicated facility for the storage of preserved samples and the supply of Liquid Nitrogen to them. These have generally undergone extensive design and feature best practise in risk management. Typical features of a dedicated cryoroom are:

• External storage of Liquid Nitrogen.

• A transfer pipeline for Liquid Nitrogen into the cryoroom – this is often referred to as Super Insulated Vacuum Line (SIVL) as it is highly insulated to prevent Liquid Nitrogen boil-off.

• The use of large cryogenic freezers, generally storing samples in vapour phase (reduces the amount of Liquid Nitrogen contained) with automated temperature control and auto-fill systems. The latter means that personnel do not have to be present when the freezers are being filled; and systems can operate on a time or level basis.

• An integrated fixed gas detection system, often with multiple sensors and alarms linked to a Business Management System and a safety shut off solenoid for the SIVL line to cut-off the Liquid Nitrogen supply in the event of an oxygen depletion alarm.

• A ventilation system linked to the gas detection equipment, providing increased throughput on low oxygen levels.

• A door interlock system that allows escape but prevent access in emergency conditions.

• Specialised flooring and lifting equipment.

• An extensive maintenance routine covering all aspects of the system.

Insert Picture of Lab tech at John Radcliffe Hospital “A typical cryoroom facility”

The second type is where Liquid Nitrogen is used in individual laboratories from small wheeled tanks or from unpressurised dewars. Liquid Nitrogen is generally manually decanted using a hose or transfer device into small aluminium freezers with liquid phase storage.

Whilst this arrangement is not as desirable as the purpose-built cryoroom, building infrastructure issues or funding often means that it is the most pragmatic way to operate. Safety can be maximised in these situations by considering the following:

• Minimise the storage of samples in a laboratory. Many facilities retain samples for archiving purposes that will rarely if ever be used in daily operation. Several private off-site repositories have opened in the UK specifically to assist with this problem.

• Minimise the amount of Liquid Nitrogen stored in your laboratory. Can the vessels be stored in a suitable outside area, where product can be transferred into a non-pressurised dewar for topping up freezers periodically?

• The use of a phase separator (a small sintered device) on liquid nitrogen transfer hoses to minimise splashing and resultant evaporation.

• The use of gas detection to allow early warning of oxygen depletion. Consider the fitment of a “repeater” or external alarm that is visible outside the laboratory to prevent people entering in emergency conditions. Fixed monitors are preferable to personal monitors as they protect everyone in an area.

• Ensuring adequate ventilation, through LEV or similar. Remember that cold nitrogen gas is heavier than air and will accumulate in low areas such as pits or gulleys.

Insert photo of CSL tanker “Liquid nitrogen is widely used and tanks are normally filled on site”.

To find out more about cryoroom and Liquid Nitrogen freezers go the CryoService Cryogenic Gases and Equipment Section

Further reading

The British Compressed Gases Association represents the UK’s industrial gas sector. It provides guidance notes and codes of practise that are recognised and utilised throughout the industry. Guidance Note GN11 “The Management of Risks Associated with Reduced Oxygen Atmospheres” has extensive additional information on this topic and is available via the BCGA website www.bcga.co.uk

Author Notes

Noor Ali

is the Sales and Marketing Manager for CryoService Limited; one of the key suppliers of liquid nitrogen, cryogenic storage systems and calibration gases in the UK. Noor has extensive experience of gas detection and liquid nitrogen applications, and has been involved in many turnkey cryoroom projects as well as taking an active stance with CoGDEM.

Andy Avenell

is the Fixed Systems Product Manager for Crowcon Detection Instruments Ltd, a market-leading manufacturer of gas detection products. Crowcon are one of the founding members of CoGDEM;the trade association representing manufacturers and service providers who are active in the field of gas detection instrumentation and environmental monitoring apparatus.

The leading Special Gases and Mixtures supplier in the UK – CryoService Ltd have today announced the appointment of Kevin Honner in their SGM division.

Kevin Honner joined CryoService in December 2008 as Business Manager (Special Gases and Mixtures) with the remit of full commercial and marketing control for all aspects of their range of speciality gases, special gas mixtures, ultra high purity gases and gas control equipment. This includes all specialist applications such as calibration gases, and special mixtures.

Kevin has had considerable experience in both the environmental monitoring and gas detection fields with over thirty years’ experience in sales, marketing and technical support for gas analysis and gas detection instrumentation, most recently as Portable Monitors Product Manager for Crowcon Detection Instruments.

More Information on the Special Gases are available on the CryoService Ltd website.

The Original Press Release is available in Special Gases and Mixtures News.

More Important industrial gas news will be following shortly.